Journal: Acta pharmacologica Sinica

Article Title: Tetrandrine alleviates silicosis by inhibiting canonical and non-canonical NLRP3 inflammasome activation in lung macrophages.

doi: 10.1038/s41401-021-00693-6

Figure Lengend Snippet: Fig. 4 Tet inhibited NLRP3 inflammasome activation by canonical and non-canonical pathways in vivo. a Functional enrichment analysis with KEGG biological processes of the significant different inflammatory protein in serum identified by quantitative inflammation microarray from control mice and silicosis mice. The top 20 pathways with a P < 0.05 were shown with their names. b Functional enrichment analysis with KEGG biological processes of the significantly different inflammatory protein in serum identified by quantitative inflammation microarray from silicosis mice treated with or without Tet. The top 20 pathways with a P < 0.05 were shown with their names. c Functional enrichment analysis with KEGG biological processes was performed using KEGG database in Si, Si + Tet, and PBS groups of mice treated as Fig. 2a (n = 3 per group). d The Venn diagram of transcriptome and quantitative inflammation microarray analysis. The intersecting signaling pathways were listed in the table in Fig. 4e. f-h The mRNA levels of TLR4, MyD88, and Caspase-11 of murine lung tissue in four groups (PBS + Vehicle, PBS + Tet, Si + Vehicle, and Si + Tet groups) treated as Fig. 2a (n = 9 per group). i Western blots of TLR4, MyD88, Pro-Caspase-11 and Cleaved Caspase-11 of murine lung tissue treated as (f). j Statistical analysis of band densities from images in (i). The data are reported as the mean ± SEM. Significance for each figure: **P < 0.01, ***P < 0.001 and ns indicates not significant.

Article Snippet: The profiles of 308 cytokines in serum of mice in blank PBS group, silica group and silica + Tet group were simultaneously detected using a mouse L308 quantitative microarray (AAM-BLG1-4, RayBiotech, Guangzhou, China) according to the manufacturer’s instructions.

Techniques: Activation Assay, In Vivo, Functional Assay, Microarray, Control, Protein-Protein interactions, Western Blot